Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_47
Snippet: While still on the magnet stand add 200 µL 80% ethanol. • Withdraw the liquid with a pipette to the bottom of the tube, and add 200 µL 80% ethanol. • Withdraw the liquid and after a quick spin, remove the remaining liquid with a 20 µL pipette. Do not air-dry the beads, but proceed immediately to the next step. • Remove from the magnet stand, add 22 µL 10 mM Tris-HCl pH 8 and vortex on full. Let sit at least 5 min. • Place on the magne.....
Document: While still on the magnet stand add 200 µL 80% ethanol. • Withdraw the liquid with a pipette to the bottom of the tube, and add 200 µL 80% ethanol. • Withdraw the liquid and after a quick spin, remove the remaining liquid with a 20 µL pipette. Do not air-dry the beads, but proceed immediately to the next step. • Remove from the magnet stand, add 22 µL 10 mM Tris-HCl pH 8 and vortex on full. Let sit at least 5 min. • Place on the magnet stand and allow to clear. • Remove the liquid to a fresh 1.5 ml tube with a pipette.
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