Author: Carossino, Mariano; Ip, Hon S.; Richt, Juergen A.; Shultz, Kendra; Harper, Kimberly; Loynachan, Alan T.; Del Piero, Fabio; Balasuriya, Udeni B. R.
Title: Detection of SARS-CoV-2 by RNAscope(®)in situ hybridization and immunohistochemistry techniques Cord-id: sk9xyd1r Document date: 2020_8_5
ID: sk9xyd1r
Snippet: In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
Document: In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope(®)-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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