Selected article for: "amplification kit and PCR amplification"
Author: A. Bal; M. Pichon; C. Picard; JS. Casalegno; M. Valette; I. Schuffenecker; L. Billard; S. Vallet; G. Vilchez; V. Cheynet; G. Oriol; S. Assant; Y. Gillet; B. Lina; K. Brengel-Pesce; F. Morfin; L. Josset
Title: Quality control implementation for universal characterization of DNA and RNA viruses in clinical respiratory samples using single metagenomic next-generation sequencing workflow
  • Document date: 2018_7_11
    • ID: d86o6j2s_13
    Snippet: The reagent cost of this mNGS approach is relatively low and was estimated to ~€150 thanks 307 to our viral enrichment process and the amplification method using a commercial kit which is 308 diluted 5-fold [21] . The use of a universal workflow for both DNA and RNA viruses also 309 reduces the reagent cost compared with metagenomic protocols targeting DNA and RNA 310 viruses separately. In contrast, targeted NGS of specific viruses following t.....
    Document: The reagent cost of this mNGS approach is relatively low and was estimated to ~€150 thanks 307 to our viral enrichment process and the amplification method using a commercial kit which is 308 diluted 5-fold [21] . The use of a universal workflow for both DNA and RNA viruses also 309 reduces the reagent cost compared with metagenomic protocols targeting DNA and RNA 310 viruses separately. In contrast, targeted NGS of specific viruses following their specific 311 amplification by PCR can be up to 2 times cheaper based on our experience (e.g. influenza 312

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