Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_40
Snippet: • To the PCR tube containing the bead slurry add 15 µL 0.67% Triton neutralization solution + 2 µL of 10 µM Universal or barcoded i5 primer + 2 µL of 10 µM uniquely barcoded i7 primers, using a different barcode for each sample. Vortex on full and place tubes in metal tube holder on ice. Tip: Indexed primers are described by Buenrostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523:4.....
Document: • To the PCR tube containing the bead slurry add 15 µL 0.67% Triton neutralization solution + 2 µL of 10 µM Universal or barcoded i5 primer + 2 µL of 10 µM uniquely barcoded i7 primers, using a different barcode for each sample. Vortex on full and place tubes in metal tube holder on ice. Tip: Indexed primers are described by Buenrostro, J.D. et al. Single-cell chromatin accessibility reveals principles of regulatory variation. Nature 523:486 (2015). Do not use Nextera or NEB primers. • Add 25 µL NEBnext (non-hot-start), vortex to mix, followed by a quick spin.
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