Author: wei zhao; Nian liu; Xiao Li Tao; Chun hong Zheng; Xiang yu Li; Man Yu; Yong gang Li
Title: Host protein CD63 enhances viral RNA replication by interacting with human astrovirus nonstructural protein nsP1a/4 Document date: 2018_5_27
ID: 8r1zb4oc_6
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint between CD63 and nsP1a by GSTpull-down analysis. We immobilized 135 nsP1a-GST on glutathione agarose and added CD63-His to assess 136 protein-protein binding. We used an untreated gel as a negative control. We 137 analyzed the protein in the complexes by western blot with anti-GST or anti-His 138 antibody. Anti-GST MA.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint between CD63 and nsP1a by GSTpull-down analysis. We immobilized 135 nsP1a-GST on glutathione agarose and added CD63-His to assess 136 protein-protein binding. We used an untreated gel as a negative control. We 137 analyzed the protein in the complexes by western blot with anti-GST or anti-His 138 antibody. Anti-GST MAb pulled down nsP1a together with CD63 (Fig. 3C ). protein in E. coli and recovered and purified the recombinant protein from the 154 bacteria (Fig 4C) . 155 We amplified the four products of the nsP1a polyprotein (nsP1a/1, nsP1a/2, 156 author/funder. All rights reserved. No reuse allowed without permission.
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