Author: Orlova, O V; Glazkova, D V; Tsyganova, G M; Antoshkina, I V; Mintaev, R R; Tikhonov, A S; Bogoslovskaya, E V; Shipulin, G A
Title: Application of real-time PCR to significantly reduce the time to obtain recombinant MVA virus. Cord-id: q89pbz06 Document date: 2020_12_24
ID: q89pbz06
Snippet: Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.
Document: Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.
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