Selected article for: "amplification step and detection limit"
Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection
  • Document date: 2020_1_16
    • ID: 8kced06y_10
    Snippet: Taken together, we demonstrate that programmable DNA nanoswitches can be developed into a robust viral RNA detection platform. The platform has key advantages over existing methodologies in terms of selectivity and specificity, as shown in our experiments with ZIKV and closely related DENV, as well as two closely related ZIKV strains. Moreover, DNA nanoswitch viral RNA detection strategy has femtomolar detection limit without an RNA amplification.....
    Document: Taken together, we demonstrate that programmable DNA nanoswitches can be developed into a robust viral RNA detection platform. The platform has key advantages over existing methodologies in terms of selectivity and specificity, as shown in our experiments with ZIKV and closely related DENV, as well as two closely related ZIKV strains. Moreover, DNA nanoswitch viral RNA detection strategy has femtomolar detection limit without an RNA amplification step, and attomolar detection limit when used with amplification. These limits are within a clinically relevant range and therefore our DNA nanoswitch assay together with the bufferless gel cartridge presents a putative diagnostic assay for clinical detection of ZIKV in low resource areas without significant laboratory infrastructure.

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