Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection Document date: 2020_1_16
ID: 8kced06y_25
Snippet: The total detection sample volume was 10 µl with 10 mM MgCl2, 1×PBS, 1.5-2 µl nanoswitch at 0.1 nM. The detection incubation was finished in a thermal cycler with a thermal annealing from 40 °C to 25 °C at -1 °C min −1 or room temperature (e.g. the NASBA related detections). Before loading to the gel, the sample was stained by GelRed (Biotium Inc.) at 1×concentration (3.3×GelRed for total RNA related detection) and mixed with 2 µl 6× .....
Document: The total detection sample volume was 10 µl with 10 mM MgCl2, 1×PBS, 1.5-2 µl nanoswitch at 0.1 nM. The detection incubation was finished in a thermal cycler with a thermal annealing from 40 °C to 25 °C at -1 °C min −1 or room temperature (e.g. the NASBA related detections). Before loading to the gel, the sample was stained by GelRed (Biotium Inc.) at 1×concentration (3.3×GelRed for total RNA related detection) and mixed with 2 µl 6× Blue loading dye.
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