Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection Document date: 2020_1_16
ID: 8kced06y_32
Snippet: Here, the amount of human urine can be scaled up as needed according to the protocol of the kit. Finally, the viral RNA was eluted from the filter column by using 15 µl nuclease-free water. Then 5 µl extracted RNA was fragmented at 94°C for 9 minutes by using 0.2× fragmentation buffer (NEB) before conducting the nanoswitch detection. Here, we lowered the use of fragmentation buffer in the consideration of the small amount of RNA in the extrac.....
Document: Here, the amount of human urine can be scaled up as needed according to the protocol of the kit. Finally, the viral RNA was eluted from the filter column by using 15 µl nuclease-free water. Then 5 µl extracted RNA was fragmented at 94°C for 9 minutes by using 0.2× fragmentation buffer (NEB) before conducting the nanoswitch detection. Here, we lowered the use of fragmentation buffer in the consideration of the small amount of RNA in the extracted sample as we noticed that too much fragmentation buffer could destroy the DNA nanoswitches (data not shown).
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