Selected article for: "sequence coverage and viral genome"
Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
  • Document date: 2020_4_20
    • ID: kyoa5gsf_42
    Snippet: We used two fully synthetic RNAs made by in vitro transcription (IVT) from Twist Biosciences, which were synthesized in 5kb pieces with full viral genome coverage of SARS-CoV-2. They were sequence verified to ensure >99.9% viral genome coverage, and come as 1,000,000 copies per µL, 100µL per tube. The two controls are from Wuhan, China (MN908947.3) and Melbourne, Australia (MT007544.1). Reference sequence designs came from NCBI: https://www.ncb.....
    Document: We used two fully synthetic RNAs made by in vitro transcription (IVT) from Twist Biosciences, which were synthesized in 5kb pieces with full viral genome coverage of SARS-CoV-2. They were sequence verified to ensure >99.9% viral genome coverage, and come as 1,000,000 copies per µL, 100µL per tube. The two controls are from Wuhan, China (MN908947.3) and Melbourne, Australia (MT007544.1). Reference sequence designs came from NCBI: https://www.ncbi.nlm.nih.gov/nuccore/MT007544 and https://www.ncbi.nlm.nih.gov/nuccore/MN908947.3.

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