PLANT-Dx: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens

M. Verosloff; J. Chappell; K. L. Perry; J. R. Thompson; J. B. Lucks

We next set out to determine whether this methodology was able to differentiate between plant lysate obtained from healthy plants versus lysate infected with CMV virus. To test this, we input 1 uL of CMV-infected plant lysate, or an equivalent volume of a non-infected plant lysate control, into the PLANT-Dx reaction system. Here, we observed rapid color change only from reactions with infected lysate when compared to healthy lysate (Figure 2A) . To demonstrate that this assay can be monitored by eye, reactions were carried out and filmed within a 31áµ’C incubator ( Figure 2B) . With the naked eye, we detected accumulation of a yellow color only within reactions that were incubated with infected lysate, while no such production was witnessed in reactions with uninfected lysate.