Author: M. Verosloff; J. Chappell; K. L. Perry; J. R. Thompson; J. B. Lucks
Title: PLANT-Dx: A Molecular Diagnostic for Point of Use Detection of Plant Pathogens Document date: 2018_12_17
ID: bzusp0lh_9
Snippet: We next set out to determine whether this methodology was able to differentiate between plant lysate obtained from healthy plants versus lysate infected with CMV virus. To test this, we input 1 uL of CMV-infected plant lysate, or an equivalent volume of a non-infected plant lysate control, into the PLANT-Dx reaction system. Here, we observed rapid color change only from reactions with infected lysate when compared to healthy lysate (Figure 2A) . .....
Document: We next set out to determine whether this methodology was able to differentiate between plant lysate obtained from healthy plants versus lysate infected with CMV virus. To test this, we input 1 uL of CMV-infected plant lysate, or an equivalent volume of a non-infected plant lysate control, into the PLANT-Dx reaction system. Here, we observed rapid color change only from reactions with infected lysate when compared to healthy lysate (Figure 2A) . To demonstrate that this assay can be monitored by eye, reactions were carried out and filmed within a 31áµ’C incubator ( Figure 2B) . With the naked eye, we detected accumulation of a yellow color only within reactions that were incubated with infected lysate, while no such production was witnessed in reactions with uninfected lysate.
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