Author: Roni Ben-Ami; Agnes Klochendler; Matan Seidel; Tal Sido; Ori Gurel-Gurevich; Moran Yassour; Eran Meshorer; Gil Benedek; Irit Fogel; Esther Oiknine-Djian; Asaf Gertler; Zeev Rotstein; Bruno Lavi; Yuval Dor; Dana G Wolf; Maayan Salton; Yotam Drier
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Document date: 2020_4_22
ID: 5s03f0pp_1
Snippet: An emerging novel severe acute respiratory syndrome-related coronavirus, SARS-CoV-2, is the virus behind the global COVID-19 pandemic. Among the foremost priorities to facilitate efficient public health interventions is a reliable and accessible diagnosis of an active SARS-CoV-2 infection. The standard laboratory diagnosis of COVID-19 involves three main steps, namely, viral inactivation and lysis of the nasopharyngeal swab sample, extraction (or.....
Document: An emerging novel severe acute respiratory syndrome-related coronavirus, SARS-CoV-2, is the virus behind the global COVID-19 pandemic. Among the foremost priorities to facilitate efficient public health interventions is a reliable and accessible diagnosis of an active SARS-CoV-2 infection. The standard laboratory diagnosis of COVID-19 involves three main steps, namely, viral inactivation and lysis of the nasopharyngeal swab sample, extraction (or purification) of viral RNA, and reverse transcription (RT)-PCR. Due to the rapid spread of the virus and the increasing demand for tests, the limited availability of test reagents, mainly RNA extraction kits, has become (and is likely to continue to be) a major bottleneck as the pandemic expands.
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