Selected article for: "cell cell heterogeneity and high resolution"

Author: Yu, Xiu-Wei; Li, Tong-Tong; Du, Xiao-Min; Shen, Qiao-Yan; Zhang, Meng-Fei; Wei, Yu-Dong; Yang, Dong-Hui; Xu, Wen-Jing; Chen, Wen-Bo; Bai, Chun-Ling; Li, Xue-Ling; Li, Guang-Peng; Li, Na; Peng, Sha; Liao, Ming-Zhi; Hua, Jin-Lian
Title: Single-cell RNA sequencing reveals atlas of dairy goat testis cells.
  • Cord-id: t28hta0n
  • Document date: 2021_7_18
  • ID: t28hta0n
    Snippet: Single-cell RNA sequencing (scRNA-seq) is useful for exploring cell heterogeneity. For large animals, however, little is known regarding spermatogonial stem cell (SSC) self-renewal regulation, especially in dairy goats. In this study, we described a high-resolution scRNA-seq atlas derived from a dairy goat. We identified six somatic cell and five spermatogenic cell subtypes. During spermatogenesis, genes with significantly changed expression were mainly enriched in the Notch, TGF-β, and Hippo s
    Document: Single-cell RNA sequencing (scRNA-seq) is useful for exploring cell heterogeneity. For large animals, however, little is known regarding spermatogonial stem cell (SSC) self-renewal regulation, especially in dairy goats. In this study, we described a high-resolution scRNA-seq atlas derived from a dairy goat. We identified six somatic cell and five spermatogenic cell subtypes. During spermatogenesis, genes with significantly changed expression were mainly enriched in the Notch, TGF-β, and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency. We detected and screened specific candidate marker genes ( TKTL1 and AES) for spermatogonia. Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells.

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