Author: Xiaotian Tan; Cory Lin; Jie Zhang; Maung Kyaw Khaing Oo; Xudong Fan
Title: Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples Document date: 2020_4_22
ID: 0tdfvlqd_13
Snippet: In the first step of the reactor preparation process ( Figure S2 (A)), the working solution of the capture antibody (i.e., D001 in S1 detection experiments) or the anti-His antibody (in IgG detection and antibody affinity experiments) were prepared by diluting the stock solution with the ELISA coating buffer (1× PBS, pH = 7.4) to achieve final concentrations of 10 μg/mL. Note that for the anti-S1 IgG detection and antibody affinity experiments,.....
Document: In the first step of the reactor preparation process ( Figure S2 (A)), the working solution of the capture antibody (i.e., D001 in S1 detection experiments) or the anti-His antibody (in IgG detection and antibody affinity experiments) were prepared by diluting the stock solution with the ELISA coating buffer (1× PBS, pH = 7.4) to achieve final concentrations of 10 μg/mL. Note that for the anti-S1 IgG detection and antibody affinity experiments, the second incubation step was used for blocking plus S1 protein immobilization (with 2 μg/mL of S1-His protein dissolved in 1% BSA).
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