Author: Yap, Tami; Khor, Shuan; Kim, Jung Seo; Kim, Jaeyoung; Kim, Sung Yun; Kern, Johannes S; Martyres, Raymond; Varigos, George; Chan, Hiu Tat; McCullough, Michael J; Thomas, Melissa L; Scardamaglia, Laura
Title: Intraoral Human Herpes Viruses Detectable by PCR in Majority of Patients. Cord-id: to952oti Document date: 2020_7_1
ID: to952oti
Snippet: OBJECTIVES To identify factors which influence the intraoral prevalence of human herpes viruses using mucosal swabs, saliva samples and qPCR analysis. METHODOLOGY In this cross-sectional observational study, matched saliva and oral swabs were collected from a total of 115 subjects: 70 immunocompetent subjects with no mucosal abnormalities, 22 with mucosal abnormalities and 23 therapeutically immunocompromised individuals. Extracted DNA was analysed by multiplex qPCR for detection and quantificat
Document: OBJECTIVES To identify factors which influence the intraoral prevalence of human herpes viruses using mucosal swabs, saliva samples and qPCR analysis. METHODOLOGY In this cross-sectional observational study, matched saliva and oral swabs were collected from a total of 115 subjects: 70 immunocompetent subjects with no mucosal abnormalities, 22 with mucosal abnormalities and 23 therapeutically immunocompromised individuals. Extracted DNA was analysed by multiplex qPCR for detection and quantification of human herpes viruses 1-6. RESULTS At least one human herpes virus was detected in 77.1% of immunocompetent individuals with no mucosal abnormalities, with EBV the most commonly detected at 61.4%. HHV-6 was detected in 17.1%, HSV-1 in 4.3% and CMV in 1.1%. Detection was higher in saliva than in oral swabs. There was no detection of HSV-2 or VZV. Neither presence of oral mucosal abnormality nor therapeutic immunocompromise was related to increased detection of human herpes virus. CONCLUSION Commensal detection rates of EBV are high and caution in clinical correlation of positive detection is warranted. Commensal CMV rates are low and detection is likely to be clinically relevant. This study presents a comprehensive commensal detection rate of human herpes viruses 1-6 by qPCR in saliva and swabs.
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