Selected article for: "amplification efficiency and detection limit"
Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler
  • Document date: 2020_4_20
    • ID: e1oinu71_22
    Snippet: The limit of detection (LoD) was determined by plotting quantification cycle (Cq) against log10 copy number concentration. Correlation coefficient (R 2 ) was calculated by linear regression analysis. Amplification efficiency (E) was calculated from the slope of the log-linear curve using the given equation: E = -1+10 (-1/slope) . The slopes and intercepts of the linear regression lines were tested for statistical significance using Analysis of Co.....
    Document: The limit of detection (LoD) was determined by plotting quantification cycle (Cq) against log10 copy number concentration. Correlation coefficient (R 2 ) was calculated by linear regression analysis. Amplification efficiency (E) was calculated from the slope of the log-linear curve using the given equation: E = -1+10 (-1/slope) . The slopes and intercepts of the linear regression lines were tested for statistical significance using Analysis of Covariance (ANCOVA) (GraphPad Prism 6). Repeatability and reproducibility of the RT-qPCR assay was determined by analysing the mean values and standard deviations of Cq values.

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