Selected article for: "direct pcr and viral nucleic acid"
Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler
  • Document date: 2020_4_20
    • ID: e1oinu71_36
    Snippet: Quantitation of viral nucleic acid in samples by RT-qPCR has been used in studies to correlate viral load with severity, prognosis and transmissibility. The Cq values in patients' samples can range from 19 to late cycles close to 40 during the course of infection. The nasal viral load has been shown to peak within days of symptom onset according to small study of throat and nasal swabs from 17 patients (29) . Using RNA as template, our DIRECT-PCR.....
    Document: Quantitation of viral nucleic acid in samples by RT-qPCR has been used in studies to correlate viral load with severity, prognosis and transmissibility. The Cq values in patients' samples can range from 19 to late cycles close to 40 during the course of infection. The nasal viral load has been shown to peak within days of symptom onset according to small study of throat and nasal swabs from 17 patients (29) . Using RNA as template, our DIRECT-PCR assay has a dynamic range of over 7 orders of magnitude with mean Cq range from 17 to 38, indicating it is reproducibly quantitative over a wide range, including very high viral titers. This method could be useful in future studies, such as to assess viral survivability, decontamination methods and preventive intervention.

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