Selected article for: "anti mouse and flow cytometry"
Author: Yujun Li; Haimin Wang; Xiaojuan Tang; Danting Ma; Chengzhi Du; Yifei Wang; Hong Pan; Qing Zou; Jie Zheng; Liangde Xu; Michael Farzan; Guocai Zhong
Title: Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1
  • Document date: 2020_4_11
    • ID: dblrxlt1_17
    Snippet: with RBD-mouse IgG2 Fc fusion proteins of SARS-CoV-2 WHU01, Pangolin-CoV, Bat-CoV RaTG13, and SARS-CoV-1 BJ01. The cells were then stained with Alexa 488 goat anti-mouse IgG secondary antibody and RBD-ACE2 binding was detected using flow cytometry. #, based on ACE2 protein sequence alignment shown in the Extended Data Figure 1 , around 20 residues (246-268) of donkey ACE2 are missing in the sequence used in this study (NCBI Reference Sequence: XM.....
    Document: with RBD-mouse IgG2 Fc fusion proteins of SARS-CoV-2 WHU01, Pangolin-CoV, Bat-CoV RaTG13, and SARS-CoV-1 BJ01. The cells were then stained with Alexa 488 goat anti-mouse IgG secondary antibody and RBD-ACE2 binding was detected using flow cytometry. #, based on ACE2 protein sequence alignment shown in the Extended Data Figure 1 , around 20 residues (246-268) of donkey ACE2 are missing in the sequence used in this study (NCBI Reference Sequence: XM_014857647.1). b, Percentages of cells positive for RBD binding in panel a are presented as a heatmap according to the indicated color code. c, Expression levels of the indicated ACE2 genes were detected using Western Blot. Data shown are representative of two independent experiments with similar results. author/funder. All rights reserved. No reuse allowed without permission.

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