Author: Yujun Li; Haimin Wang; Xiaojuan Tang; Danting Ma; Chengzhi Du; Yifei Wang; Hong Pan; Qing Zou; Jie Zheng; Liangde Xu; Michael Farzan; Guocai Zhong
Title: Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1 Document date: 2020_4_11
ID: dblrxlt1_17
Snippet: with RBD-mouse IgG2 Fc fusion proteins of SARS-CoV-2 WHU01, Pangolin-CoV, Bat-CoV RaTG13, and SARS-CoV-1 BJ01. The cells were then stained with Alexa 488 goat anti-mouse IgG secondary antibody and RBD-ACE2 binding was detected using flow cytometry. #, based on ACE2 protein sequence alignment shown in the Extended Data Figure 1 , around 20 residues (246-268) of donkey ACE2 are missing in the sequence used in this study (NCBI Reference Sequence: XM.....
Document: with RBD-mouse IgG2 Fc fusion proteins of SARS-CoV-2 WHU01, Pangolin-CoV, Bat-CoV RaTG13, and SARS-CoV-1 BJ01. The cells were then stained with Alexa 488 goat anti-mouse IgG secondary antibody and RBD-ACE2 binding was detected using flow cytometry. #, based on ACE2 protein sequence alignment shown in the Extended Data Figure 1 , around 20 residues (246-268) of donkey ACE2 are missing in the sequence used in this study (NCBI Reference Sequence: XM_014857647.1). b, Percentages of cells positive for RBD binding in panel a are presented as a heatmap according to the indicated color code. c, Expression levels of the indicated ACE2 genes were detected using Western Blot. Data shown are representative of two independent experiments with similar results. author/funder. All rights reserved. No reuse allowed without permission.
Search related documents:
Co phrase search for related documents- fusion protein and RBD bind: 1, 2, 3, 4, 5, 6, 7
- fusion protein and RBD mouse: 1, 2, 3
- fusion protein and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8
- fusion protein and similar result: 1
- fusion protein and study sequence: 1, 2, 3
- fusion protein and Western Blot: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57
- fusion protein and Western Blot detect: 1, 2
- IgG secondary antibody and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47
- IgG secondary antibody and Western Blot: 1
- independent experiment and secondary antibody: 1
- independent experiment and Western Blot: 1
- RBD bind and study sequence: 1
- secondary antibody and similar result: 1
- secondary antibody and Western Blot: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27
- secondary antibody and Western Blot detect: 1, 2, 3
- similar result and Western Blot: 1, 2, 3, 4, 5
- study sequence and Western Blot: 1, 2
Co phrase search for related documents, hyperlinks ordered by date