Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Document date: 2020_4_20
ID: e1oinu71_15
Snippet: The lowest limit of detection (LoD) was determined using SARS-CoV-2 N RNA template that was ten-fold serially diluted in SARS-CoV-2-negative sputum and nasal exudate samples. LoD was defined as the last dilution in which quantification cycle (Cq) value could be detected in all replicates. The linear range correlation between the theoretical log copy number calculated from the concentration molarity of synthetic nucleic acids and Cq value was esta.....
Document: The lowest limit of detection (LoD) was determined using SARS-CoV-2 N RNA template that was ten-fold serially diluted in SARS-CoV-2-negative sputum and nasal exudate samples. LoD was defined as the last dilution in which quantification cycle (Cq) value could be detected in all replicates. The linear range correlation between the theoretical log copy number calculated from the concentration molarity of synthetic nucleic acids and Cq value was established applying a best-fit line to the data by linear regression analysis. PCR efficiency (E) was calculated from the slope of the linear equation.
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