Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Document date: 2020_4_20
ID: e1oinu71_20
Snippet: DIRECT-PCR assays were validated on the portable thermocycler (MyGo Mini, IT-IS Life Science Ltd, Ireland) using the same monoplex single-tube protocols described above. The portable thermocycler is lightweight at less than 2 kg with a dimension of 12 cm (width) by 12 cm (depth) by 16 cm (height). It can perform up to 16 reactions using standard 0.1 mL clear qPCR tubes with 10 to 100 µL reaction volume each. The performance of this portable qPCR.....
Document: DIRECT-PCR assays were validated on the portable thermocycler (MyGo Mini, IT-IS Life Science Ltd, Ireland) using the same monoplex single-tube protocols described above. The portable thermocycler is lightweight at less than 2 kg with a dimension of 12 cm (width) by 12 cm (depth) by 16 cm (height). It can perform up to 16 reactions using standard 0.1 mL clear qPCR tubes with 10 to 100 µL reaction volume each. The performance of this portable qPCR thermocycler was evaluated by comparing the LoD and amplification efficiency (E) of DIRECT-PCR assays.
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