Author: Steven Henikoff; Jorja G. Henikoff
Title: Profiling the epigenome at home Document date: 2020_4_17
ID: mojf4l6b_50
Snippet: . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.043083 doi: bioRxiv preprint • Perform paired-end Illumina sequencing on the barcoded libraries following the manufacturer's instructions. For maximum economy, paired-end PE25 is more than sufficient for mapping to large genomes. • Using paired-end 25x25 .....
Document: . CC-BY 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.15.043083 doi: bioRxiv preprint • Perform paired-end Illumina sequencing on the barcoded libraries following the manufacturer's instructions. For maximum economy, paired-end PE25 is more than sufficient for mapping to large genomes. • Using paired-end 25x25 sequencing on a HiSeq 2-lane rapid run flow cell we obtain ~300 million total mapped reads, or ~3 million per sample when there are 96 samples mixed to obtain approximately equal molarity.
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