Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Document date: 2020_4_20
ID: e1oinu71_8
Snippet: Approximately 1 mL of SARS-CoV-2-negative sputum and nasal exudate from a healthy adult research team member was collected by hypersaline inhalation, and used for spike-in of ssRNA and plasmid controls. Briefly, the respiratory samples were mixed in 1:1 (v/v) ratio with Sputasol (Oxoid, England) and vortexed for 5 minutes to remove the viscosity to allow direct addition to the PCR reaction mix......
Document: Approximately 1 mL of SARS-CoV-2-negative sputum and nasal exudate from a healthy adult research team member was collected by hypersaline inhalation, and used for spike-in of ssRNA and plasmid controls. Briefly, the respiratory samples were mixed in 1:1 (v/v) ratio with Sputasol (Oxoid, England) and vortexed for 5 minutes to remove the viscosity to allow direct addition to the PCR reaction mix.
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