Selected article for: "free water and nuclease free water"
Author: Lifeng Zhou; Arun Richard Chandrasekaran; Jibin Abraham Punnoose; Gaston Bonenfant; Stephon Charles; Oksana Levchenko; Pheonah Badu; Cassandra Cavaliere; Cara T. Pager; Ken Halvorsen
Title: Programmable low-cost DNA-based platform for viral RNA detection
  • Document date: 2020_1_16
    • ID: 8kced06y_29
    Snippet: Detection of viral RNA from total RNA First, 500 ng total RNA extracted from uninfected/infected cells was fragmented at 94°C for 9 minutes in 1x fragmentation buffer. Fragmented total RNA was then mixed with nanoswitches (1 nM), MgCl 2 (10 mM) and PBS (1×), and the mixture was made up to 10 µl with nuclease-free water. Samples were then incubated in a thermal annealing ramp from 40 °C to 25 °C at 1 °C min −1 . After the incubation, sampl.....
    Document: Detection of viral RNA from total RNA First, 500 ng total RNA extracted from uninfected/infected cells was fragmented at 94°C for 9 minutes in 1x fragmentation buffer. Fragmented total RNA was then mixed with nanoswitches (1 nM), MgCl 2 (10 mM) and PBS (1×), and the mixture was made up to 10 µl with nuclease-free water. Samples were then incubated in a thermal annealing ramp from 40 °C to 25 °C at 1 °C min −1 . After the incubation, samples were stained with GelRed (Biotium Inc.) at 3.3× concentration and incubated at room temperature for 30 min. Before loading the gel, 2 µl of 6x blue loading dye was mixed with each sample, and 10 µl sample was loaded to each well. Samples were run in a 0.8% agarose gel at 65-75 V for about 70-90 minutes in the cold room.

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