Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_6
Snippet: Briefly, a pan IBV RT-qPCR assay 4 was used to detect IBV in general and then IBV strain specific RT-qPCR assay 36 was used to detect GA08 in the samples using the AgPath ID, One step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Applied Biosystems 7500 FAST following the previously described protocols......
Document: Briefly, a pan IBV RT-qPCR assay 4 was used to detect IBV in general and then IBV strain specific RT-qPCR assay 36 was used to detect GA08 in the samples using the AgPath ID, One step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Applied Biosystems 7500 FAST following the previously described protocols.
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