Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_61
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/634600 doi: bioRxiv preprint target sequences. 28 Additionally, it is possible that certain genotypes are better complemented to the S1 primers than others and may outcompete those isolates for amplification in the AmpSeq protocol. Lastly, increasing the total number of reads collected by AmpSeq may improve the ability to detect al.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/634600 doi: bioRxiv preprint target sequences. 28 Additionally, it is possible that certain genotypes are better complemented to the S1 primers than others and may outcompete those isolates for amplification in the AmpSeq protocol. Lastly, increasing the total number of reads collected by AmpSeq may improve the ability to detect all genotypes in sample by AmpSeq. It is possible to allow the sequencing to continue longer to obtain more reads per sample. Overall, AmpSeq is a feasible test for IBV characterization, and work is ongoing to improve this new type of assay.
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