Author: Alisha Chitrakar; Sneha Rath; Jesse Donovan; Kaitlin Demarest; Yize Li; Raghavendra Rao Sridhar; Susan R. Weiss; Sergei V. Kotenko; Ned S. Wingreen; Alexei Korennykh
Title: Realtime 2-5A kinetics suggests interferons ß and ? evade global arrest of translation by RNase L Document date: 2018_11_26
ID: mxdvdw9u_30
Snippet: Metabolic labeling with 35 S was conducted using the following procedure. After cell treatment with poly-IC, media was changed to methionine-free RPMI + 10% FBS supplemented with 11 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Cellular proteins were resolved by 10% BisTris PAGE (NuPAGE) and analyzed by phosphorimaging. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.or.....
Document: Metabolic labeling with 35 S was conducted using the following procedure. After cell treatment with poly-IC, media was changed to methionine-free RPMI + 10% FBS supplemented with 11 μCi EasyTag™ EXPRESS35S Protein Labeling Mix (Perkin Elmer). Cellular proteins were resolved by 10% BisTris PAGE (NuPAGE) and analyzed by phosphorimaging. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/476341 doi: bioRxiv preprint residual poly-IC. After the washes, fresh 1 mL medium was added and the cells were kept in fresh media for one hour to allow for protein secretion.
Search related documents:
Co phrase search for related documents- cell treatment and metabolic labeling: 1
- cell treatment and protein secretion: 1
- cellular protein and metabolic labeling: 1, 2, 3, 4
- cellular protein and protein secretion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- following procedure and protein secretion: 1
Co phrase search for related documents, hyperlinks ordered by date