Selected article for: "gene primer and real time"
Author: Yinhua Zhang; Nelson Odiwuor; Jin Xiong; Luo Sun; Raphael Ohuru Nyaruaba; Hongping Wei; Nathan A Tanner
Title: Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP
  • Document date: 2020_2_29
    • ID: cv3qgno3_21
    Snippet: is the (which was not peer-reviewed) The copyright holder for this preprint . Comparison of RNA and gBlock dsDNA templates in LAMP amplification using real time amplification curves. Two primer sets (ORF1a-A and Gene N-A) were used to amplify either RNA (green curves, dilutions from 120x10 6 to 120 copies) or gDNA (blue curves, 60x10 6 to 60 copies). For ORF1a-A primer set, the gBlock is faster than RNA template; For Gene N-A primer set, the RNA .....
    Document: is the (which was not peer-reviewed) The copyright holder for this preprint . Comparison of RNA and gBlock dsDNA templates in LAMP amplification using real time amplification curves. Two primer sets (ORF1a-A and Gene N-A) were used to amplify either RNA (green curves, dilutions from 120x10 6 to 120 copies) or gDNA (blue curves, 60x10 6 to 60 copies). For ORF1a-A primer set, the gBlock is faster than RNA template; For Gene N-A primer set, the RNA is slightly faster. Each "cycle" represents 20 seconds, with 30 minute timepoint noted by dashed line. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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