Selected article for: "lung tissue and primary cell"

Author: Longlong Si; Haiqing Bai; Melissa Rodas; Wuji Cao; Crystal Yur Oh; Amanda Jiang; Atiq Nurani; Danni Y Zhu; Girija Goyal; Sarah Gilpin; Rachelle Prantil-Baun; Donald E. Ingber
Title: Human organs-on-chips as tools for repurposing approved drugs as potential influenza and COVID19 therapeutics in viral pandemics
  • Document date: 2020_4_14
  • ID: mrgw2mnx_2
    Snippet: Infections by respiratory viruses are currently studied using cultured established cell lines, primary tissue-derived human cells, human organoids, ex vivo human lung tissue cultures, and animal models; however, all of these preclinical models have significant limitations (Supplementary Table 1 ) 9-11 . For example, cultured cell lines often need to be supplemented with exogenous proteases to enable viral propagation, whereas endogenous proteases.....
    Document: Infections by respiratory viruses are currently studied using cultured established cell lines, primary tissue-derived human cells, human organoids, ex vivo human lung tissue cultures, and animal models; however, all of these preclinical models have significant limitations (Supplementary Table 1 ) 9-11 . For example, cultured cell lines often need to be supplemented with exogenous proteases to enable viral propagation, whereas endogenous proteases that are naturally expressed by the airway epithelium exert this crucial action in humans 10 . Cell lines and even human cells grown in conventional cultures do not exhibit the highly differentiated tissue structures and functions seen in living human organs. Explant cultures of human respiratory tract tissue circumvent this limitation, but their availability is limited and their viability can only be maintained for short time (4-10 days) 11 . While human lung organoids provide a more author/funder. All rights reserved. No reuse allowed without permission.

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