Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry

Yingyun Cai; Shuiqing Yu; Rohit Jangra; Elena Postnikova; Jiro Wada; Robert Tesh; Sean Whelan; Michael Lauck; Michael Wiley; Courtney Finch; Sheli Radoshitzky; David O'Connor; Gustavo Palacios; Kartik Chandran; Charles Chiu; Jens Kuhn

Tibrovirus cell entry 11 viruses. Cultured isolates of BASV, EKV-1, and EKV-2 are not available and, therefore, could 414 not be included in the experiment. 415 The large size of the experiment and the absence of commercially available or generally four tibroviruses in exposed Asian tiger mosquito and Vero cells. As expected, anti-TIBV N 422 antibody detected TIBV in these TIBV-infected cells. Anti-CPV N antibody detected CPV in 423 CPV-exposed Asian tiger mosquito cells but not in Vero cells. Encouragingly, anti-TIBV N 424 antibody also detected BAV (but not CPV and only weakly SWBV), and anti-CPV N antibody 425 also detected SWBV (but not TIBV and only very weakly BAV) ( Figure 5A ). 426 We next exposed all human, bat, nonhuman primate, hispid cotton rat, boa constrictor, (Table 443 2). In nonhuman cell lines, N was undetectable in cell lines that did not develop CPE with the 444 exception of four non-human primate cell lines, which expressed N despite the absence of CPE 445 (Table 3 ). In contrast, CPE and N detection was largely absent in all cell lines infected with The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/507350 doi: bioRxiv preprint