Author: Guido Papa; Luca Venditti; Francesca Arnoldi; Elisabeth M. Schraner; Christiaan Potgieter; Alexander Borodavka; Catherine Eichwald; Oscar R. Burrone
Title: Recombinant rotaviruses rescued by reverse genetics reveal the role of NSP5 hyperphosphorylation in the assembly of viral factories Document date: 2019_6_4
ID: 1xdrd4dd_8
Snippet: Newly synthesized RNAs were labeled by including 2 mM 5-ethynyl uridine (EU) 302 into the cell culture medium, and modified incorporated nucleotides were reacted 303 with an azide-conjugated fluorophore Alexa-488 following the manufacturer's 304 protocol for Click-iT RNA Alexa Fluor 488 imaging kit (Thermo Fisher Scientific). 305.....
Document: Newly synthesized RNAs were labeled by including 2 mM 5-ethynyl uridine (EU) 302 into the cell culture medium, and modified incorporated nucleotides were reacted 303 with an azide-conjugated fluorophore Alexa-488 following the manufacturer's 304 protocol for Click-iT RNA Alexa Fluor 488 imaging kit (Thermo Fisher Scientific). 305
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