A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterization in different cell types

Miserey-Lenkei, Stéphanie; Trajkovic, Katarina; Čopič, Alenka; Mathur, Pallavi; Schauer, Kristine; Goud, bruno; Albanèse, Véronique

Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g. Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. To facilitate further cellular investigations, notably by imaging techniques, we present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterization in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localization of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localization of Nsp13 to the centrosome, Orf3a to late endosomes, and Orf9b to mitochondria.