Author: Soon Keong Wee; Suppiah Paramalingam Sivalingam; Eric Peng Huat Yap
Title: Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Document date: 2020_4_20
ID: e1oinu71_10
Snippet: All primers and probes targeting SARS-CoV-2 ORF1ab and N genes were previously published by Chinese Center for Disease Control and Prevention, Beijing, China (24, 25) . The human Ribonuclease P (RP) gene primer and probe were previously published by US Centers for Disease Control and Prevention (CDC) protocol and was added as an internal control to detect the presence of human RNA in the samples (26) . All oligonucleotides, primers and probes wer.....
Document: All primers and probes targeting SARS-CoV-2 ORF1ab and N genes were previously published by Chinese Center for Disease Control and Prevention, Beijing, China (24, 25) . The human Ribonuclease P (RP) gene primer and probe were previously published by US Centers for Disease Control and Prevention (CDC) protocol and was added as an internal control to detect the presence of human RNA in the samples (26) . All oligonucleotides, primers and probes were commercially purchased from Integrated DNA Technologies (San Diego).
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