Prediction of SARS-CoV interaction with host proteins during lung aging reveals a potential role for TRIB3 in COVID-19

Diogo de Moraes; Brunno Vivone Buquete Paiva; Sarah Santiloni Cury; João Pessoa Araújo Junior; Marcelo Alves da Silva Mori; Robson Francisco Carvalho

The conserved evolutionary relationship between the 2019 novel SARS-CoV-2 and SARS-CoV 9 opens up the possibility to explore the relationships of these human coronaviruses (HCoVs) in publicly available databases. Thus, we compared the list of DEG in the lung during aging with corresponding human proteins that potentially interact with HCoVs (Table S2 ). The HCoVs-human PPIs were obtained using data from the Pathogen-Host Interactome Prediction using Structure Similarity (P-HIPSTer, http://phipster.org/) database, which is a comprehensive catalog of the virushuman PPIs predicted based on protein structural information 11 (Table S2) . P-HIPSTer has an experimental validation rate of ∼ 76% 11 . We also compared the list of DEG with recently added libraries for virus perturbations (up-and down-regulation) from GEO datasets (GSE33266, GSE50000, GSE49262, GSE50878, GSE49263, GSE40824, GSE50878, GSE49263, GSE47960, GSE47961, GSE47962, GSE17400, and GSE40824), available at the EnrichR database 34 . Access in March 2020. Genes that are common in either direction of both conditions were analyzed on STRING (https://string-db.org/) 35 . Access in March 2020.