Prediction of SARS-CoV interaction with host proteins during lung aging reveals a potential role for TRIB3 in COVID-19

Diogo de Moraes; Brunno Vivone Buquete Paiva; Sarah Santiloni Cury; João Pessoa Araújo Junior; Marcelo Alves da Silva Mori; Robson Francisco Carvalho

We analyzed the expression of TRIB3, HAPLN2, and ACE2, in different lung cell populations by using two previously published human single-cell RNA-seq data (Table S4) 19, 20 . The first dataset 20 was explored in the UCSC Cell Browser (http://nupulmonary.org/resources/), aiming the identification of the cell populations expressing those genes. The samples with pulmonary fibrosis presented in this dataset were omitted from our analysis, and only non-diseased lung samples were included (n=8). We further used an independent single-cell RNA-seq dataset 19 (n=5), available at the . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.07.030767 doi: bioRxiv preprint Human Cell Atlas Portal (https://data.humancellatlas.org/explore/projects/c4077b3c-5c98-4d26-a614-246d12c2e5d7), to confirm that expression TRIB3 and ACE2 are expressed in alveolar epithelial cells (types 1 and 2) and in ciliate cells. Access in March 2020.