Author: Rudo Kieft; Yang Zhang; Alexandre P. Marand; Jose Dagoberto Moran; Robert Bridger; Lance Wells; Robert J. Schmitz; Robert Sabatini
Title: Identification of a Novel Base J Binding Protein Complex Involved in RNA Polymerase II Transcription Termination in Trypanosomes Document date: 2019_8_30
ID: j3u7yq3y_1
Snippet: 240 Subsequently, the TEV protease eluate underwent anti-Protein C affinity purification, and the final purified 241 products were eluted with EGTA. The concentrated proteins were then trypsin-digested and analyzed by 242 LC-MS/MS. As a control, we analyzed the eluate of a comparable purification of extract from wild-type T. 243 brucei. As expected, TbPNUTS was identified along with Wdr82 and JBP3 (Table 2).....
Document: 240 Subsequently, the TEV protease eluate underwent anti-Protein C affinity purification, and the final purified 241 products were eluted with EGTA. The concentrated proteins were then trypsin-digested and analyzed by 242 LC-MS/MS. As a control, we analyzed the eluate of a comparable purification of extract from wild-type T. 243 brucei. As expected, TbPNUTS was identified along with Wdr82 and JBP3 (Table 2)
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