Identification of Human Immune Cell Subtypes Most Vulnerable to IL-1ß-induced Inflammatory Signaling Using Mass Cytometry

Hema Kothari; Corey M. Williams; Chantel McSkimming; Mythili Vigneshwar; Eli R. Zunder; Coleen A. McNamara

Notably, cluster 12 was found to be Linand only expressed CD38 and a low level of CXCR4. IL-1β induced the most robust expression of p-NF-kB (~13-fold higher as compared to vehicle control) in the CM cluster 1 (Fig. 4d) . In addition, a modest 2.5 to 5-fold induction in the frequency of p-NF-kB + cells was observed in the other CM clusters including 2, 6 and 9. Interestingly, of all the CM clusters, cluster 1 showed the highest median expression of CCR2 and Tollip, and was distinct from the other CM clusters in terms of lower expression of CD11b and CD45RO (Fig. 4c) . Of the two DC clusters, IL-1βinduced an increase in the frequency of p-NF-kB + cells (13 to 30-fold) within the CD11c mDCs (cluster 8) (Fig. 4d) . Despite the dramatic fold change, the increase was not statistically significant, likely due to donor heterogeneity. A modest and trending increase in the frequency of p-p38 + cells was observed within the same CM clusters (1, 2, 6 and 9) and CD11c mDCs (Fig. 4e) . In addition to monocyte subsets and CD11c mDCs, IL-1β also induced expression of p-NF-kB and p-p38 in the HLA-DR + Lincells (cluster 12).