P(2)Y(12) receptor blockers are anti-inflammatory drugs inhibiting both circulating monocytes and macrophages including THP-1 cells

Siegel, Patrick M.; Sander, Laura; Fricke, Alba; Stamm, Johannes; Wang, Xiaowei; Sharma, Prerna; Bassler, Nicole; Ying, Ya-Lan; Olivier, Christoph B.; Eisenhardt, Steffen U.; Bode, Christoph; Ahrens, Ingo; Diehl, Philipp; Peter, Karlheinz

P(2)Y(12) blockade improves patient outcomes after myocardial infarction. As well as antithrombotic effects, anti-inflammatory effects may contribute to this beneficial clinical outcome. Here we aimed to identify potential anti-inflammatory effects of P(2)Y(12) receptor blockers on monocytes and macrophages. Using flow cytometry, migration assays, flow chambers and RNA microarrays, we investigated the effects of adenosine diphosphate (ADP) and P(2)Y(12) receptor blockers on blood monocytes, THP-1 monocytes and THP-1 monocytes after differentiation to macrophages. P(2)Y(12) -expressing platelets can form aggregates with monocytes in circulating blood. Mediated by platelets, ADP results in activation of the integrin receptor Mac-1 on blood monocytes, as detected by the conformation-specific single-chain antibody MAN-1. Via the same association with platelets, THP-1 monocyte adhesion to the endothelial intercellular adhesion molecule 1 (ICAM-1) is induced by ADP. P(2)Y(12) receptor blockers prevent these ADP effects on monocytes. Interestingly, in contrast to THP-1 monocytes, THP-1 monocytes, after differentiation to macrophages, directly expressed the P(2)Y(12) receptor and consequently ADP was found to be a potent chemoattractant. Again, P(2)Y(12) receptor blockers antagonised this effect. Accordingly, stimulation of THP-1 macrophages with ADP caused a substantial change in gene expression pattern and upregulation of several genes associated with inflammation and atherogenesis. These data establish novel anti-inflammatory effects of P(2)Y(12) receptor blockers on monocytes and macrophages, which are expected to contribute to cardiovascular risk reduction.