Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics

Alpana Waghmare; Elizabeth M Krantz; Subhasish Baral; Emma Vasquez; Tillie Loeffelholz; E. Lisa Chung; Urvashi Pandey; Jane Kuypers; Elizabeth R Duke; Keith R Jerome; Alexander L Greninger; Daniel B Reeves; Florian Hladik; E Fabian Cardozo-Ojeda; Michael Boeckh; Joshua T Schiffer

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.03.20051706 doi: medRxiv preprint Viral load kinetics as a predictor of respiratory virus symptoms. In general, the level of 138 symptoms appeared to track with detectable virus, particularly for COV, HRV and MPV. For the 139 single case of RSV, a high number of symptoms persisted beyond viral elimination (Fig 2a) . For 140 all HRV infections of greater than one day, duration of shedding correlated strongly with 141 duration of symptoms (r=0.87). In these HRV infected individuals, symptoms subsided 142 immediately before, concurrent with or soon after viral elimination. Low viral load infections 143 lasting only a day were associated with a smaller number of symptoms than more prolonged 144 higher viral load episodes (Fig 2a) . 145 146 Stable and surging nasal cytokine levels during respiratory virus infection. We next 147 followed the levels of 20 different cytokines during infection measured from the same specimens 148 from which the viral load was measured. For several cytokines particularly those in the Th2, 149