Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP

Yinhua Zhang; Nelson Odiwuor; Jin Xiong; Luo Sun; Raphael Ohuru Nyaruaba; Hongping Wei; Nathan A Tanner

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org /10.1101 /10. /2020 In conclusion, colorimetric LAMP provides a simple, rapid method for SARS-CoV-2 RNA detection. Not only purified RNA can be used as the sample input, but also direct tissue or cell lysate may be used without an RNA purification step. This combination of a quick sample preparation method with an easy detection process may allow the development of portable, field detection in addition to a rapid screening for point-of-need testing applications. This virus represents an emerging significant public health concern and expanding the scope of diagnostic utility to applications outside of traditional laboratories will enable greater prevention and surveillance approaches. The efforts made here will serve as a model for inevitable future outbreaks where the use of next generation portable diagnostics will dramatically expand the reach of our testing capabilities for better healthcare outcomes.