Selected article for: "final volume and Primer sequence"
Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples
  • Document date: 2019_5_10
    • ID: hxmk6gvr_23
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/634600 doi: bioRxiv preprint and Massachusetts serotypes). Secondly, a primer set based on GA98 sequence NCBI AF274439.1 was used to amplify Genotype IV-lineage 1 viruses (e.g., Georgia 1998 and Delaware 072 serotypes). Primer sets were designed using NCBI Primer-BLAST. 51 The PCR reaction mixture is composed of 10 µl of cDNA, 0.5.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/634600 doi: bioRxiv preprint and Massachusetts serotypes). Secondly, a primer set based on GA98 sequence NCBI AF274439.1 was used to amplify Genotype IV-lineage 1 viruses (e.g., Georgia 1998 and Delaware 072 serotypes). Primer sets were designed using NCBI Primer-BLAST. 51 The PCR reaction mixture is composed of 10 µl of cDNA, 0.5 µl of 10 µM forward primer, 0.5 µl of 10 µM reverse primer, 2.5 µl of 10× buffer, 0.5 µl of enzyme mix, and 0.5 µl of 10mM dNTPs, then made the final volume to 25 µl with nuclease-free water. The following thermocycling conditions were used for amplicon synthesis: denaturation at 95 °C for 1 min, followed by

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