Imaging Striatal Dopamine Release Using a Non-Genetically Encoded Near-Infrared Fluorescent Catecholamine Nanosensor

Abraham G. Beyene; Kristen Delevich; Jackson Travis Del Bonis-O’Donnell; David J. Piekarski; Wan Chen Lin; A. Wren Thomas; Sarah J. Yang; Polina Kosillo; Darwin Yang; Linda Wilbrecht; Markita P. Landry

Schematic of optical catecholamine reporters, nIRCats. Pristine SWNT are functionalized with (GT)6 oligonucleotides to generate turn-on optical reporters for dopamine (DA) and norepinephrine (NE) (b) Fluorescence spectra of nIRCats before (black) and after (red) addition of 10 µM of dopamine in an in vitro preparation in phosphate buffered saline (without tissue). Multiple emission peaks correspond to unique SWNT chiralities contained within the multi-chirality mixture. (c) Nanosensor optical response to 100 µM dopamine (DA), norepinephrine (NE), glutamate (GLU), γ-aminobutyric acid (GABA), and acetylcholine (ACH) (data from in vitro testing). Black bars represent averages from n=3 independent measurements and error bars are calculated as standard deviations of the n=3 measurements.