Selected article for: "housekeeping gene and reverse primer"
Author: Cara E. Brook; Mike Boots; Kartik Chandran; Andrew P. Dobson; Christian Drosten; Andrea L. Graham; Bryan T. Grenfell; Marcel A. Müller; Melinda Ng; Lin-Fa Wang; Anieke van Leeuwen
Title: Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence
  • Document date: 2019_7_8
    • ID: 683qcgd9_10
    Snippet: We undertook qPCR of cDNA to assess expression of the type I interferon genes, IFN-529 and IFN-, and the housekeeping gene, -Actin, using primers previously reported in the 530 literature (Supplementary File 6). For qPCR, 2ul of each cDNA sample was incubated with 7ul 531 of deionized water, 1ul of 5UM forward/reverse primer mix and 10ul of iTaq Universal SYBR 532.....
    Document: We undertook qPCR of cDNA to assess expression of the type I interferon genes, IFN-529 and IFN-, and the housekeeping gene, -Actin, using primers previously reported in the 530 literature (Supplementary File 6). For qPCR, 2ul of each cDNA sample was incubated with 7ul 531 of deionized water, 1ul of 5UM forward/reverse primer mix and 10ul of iTaq Universal SYBR 532

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