Selected article for: "complete SARS CoV genome and SARS CoV genome"
Author: Jessica E. Manning; Jennifer A. Bohl; Sreyngim Lay; Sophana Chea; Ly Sovann; Yi Sengdoeurn; Seng Heng; Chan Vuthy; Katrina Kalantar; Vida Ahyong; Michelle Tan; Jonathan Sheu; Cristina M. Tato; Joseph L. DeRisi; Laurence Baril; Veasna Duong; Philippe Dussart; Erik A. Karlsson
Title: Rapid metagenomic characterization of a case of imported COVID-19 in Cambodia
  • Document date: 2020_3_5
    • ID: fu3cl7lq_2
    Snippet: Here, in a rapidly implemented response to the novel coronavirus outbreak, we used an unbiased metagenomics next generation sequencing (mNGS) approach on the index COVID-19 case in Cambodia as a tactical demonstration of an efficient workflow to identify and characterize the novel virus. While unbiased sequencing successfully achieved an unambiguous identification of the virus in the absence of any sequence enrichment, complete coverage of the SA.....
    Document: Here, in a rapidly implemented response to the novel coronavirus outbreak, we used an unbiased metagenomics next generation sequencing (mNGS) approach on the index COVID-19 case in Cambodia as a tactical demonstration of an efficient workflow to identify and characterize the novel virus. While unbiased sequencing successfully achieved an unambiguous identification of the virus in the absence of any sequence enrichment, complete coverage of the SARS-CoV-2 genome was subsequently achieved by augmenting the mNGS library preparation via a spiked primer enrichment approach. 6

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