Author: Alamillo, Josefa M.; Monger, Wendy; Sola, Isabel; GarcÃa, Beatriz; Perrin, Yolande; Bestagno, Marco; Burrone, Oscar R.; Sabella, Patricia; Planaâ€Durán, Joan; Enjuanes, Luis; Lomonossoff, George P.; GarcÃa, Juan A.
Title: Use of virus vectors for the expression in plants of active fullâ€length and single chain antiâ€coronavirus antibodies Cord-id: w1vyz452 Document date: 2006_9_27
ID: w1vyz452
Snippet: To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a fullâ€length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV
Document: To extend the potential of antibodies and their derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed both a small immune protein (SIP) and a fullâ€length antibody in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The agr;SIP molecule consisted of a single chain antibody (scFv) specific for the porcine coronavirus, transmissible gastroenteritis virus (TGEV) linked to the αâ€CH3 domain from human IgA. To express the fullâ€length IgA, the individual light and heavy chains from the TGEVâ€specific mAb 6A.C3 were inserted into separate PVX constructs and plants were coâ€infected with both constructs. Western blot analysis revealed the efficient expression of both the SIP and IgA molecules. Analysis of crude plant extracts revealed that both the plantâ€expressed αSIP and IgA molecules could bind to and neutralize TGEV in tissue culture, indicating that active molecules were produced. Oral administration of crude extracts from antibodyâ€expressing plant tissue to 2â€dayâ€old piglets showed that both the αSIP and fullâ€length IgA molecules can provide in vivo protection against TGEV.
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