Selected article for: "ELISA immunosorbent assay and screening test"
Author: Pieri, Massimo; Ciotti, Marco; Carlozzi, Nicoletta; Frassanito, Maria Loredana; Meloni, Arianna; Cistera, Alessandro; Turchetti, Giordano; Niscola, Silvia; Labate, Giuseppe; Calugi, Graziella; Bernardini, Sergio
Title: SARS-CoV-2 infection serology validation of different methods: Usefulness of IgA in the early phase of infection
  • Cord-id: rvcd8vvx
  • Document date: 2020_9_28
    • ID: rvcd8vvx
    Snippet: BACKGROUND AND AIMS: A novel coronavirus (SARS-CoV-2) was isolated from the respiratory samples of patients with pneumonia as showed by the sequence analysis of the virus genomes obtained in Wuhan, China. The antibody response to SARS-CoV-2 is not well understood yet, but the availability of sensitive and specific serological assays will be crucial for the early diagnosis of infection, for epidemiological studies and for defining the presence of neutralizing antibodies in response to a possible
    Document: BACKGROUND AND AIMS: A novel coronavirus (SARS-CoV-2) was isolated from the respiratory samples of patients with pneumonia as showed by the sequence analysis of the virus genomes obtained in Wuhan, China. The antibody response to SARS-CoV-2 is not well understood yet, but the availability of sensitive and specific serological assays will be crucial for the early diagnosis of infection, for epidemiological studies and for defining the presence of neutralizing antibodies in response to a possible vaccine. MATERIALS AND METHODS: We tested and compared the performances of one chemiluminescent immunoassay (CLIA), two enzyme-linked immunosorbent assay (ELISA) and an electrochemiluminescence immunoassay (ECLIA). RESULTS: The ECLIA serological assay performed best and may be a valid screening method for SARS-COV-2 infection. The IgA detected by the ELISA assay might be a more reliable and stable early serological marker than IgM. Instead, IgGs, as expected, showed stable level after 10 days from symptoms onset. CONCLUSION: The ECLIA method could be used as screening test, considering both the excellent performance and the cost per single test; while ELISA assay for IgG and IgA, which are present at a higher level than IgM and last longer, might be used as confirmatory test.

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