Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_22
Snippet: For Sanger sequencing amplicons synthesis, cDNA from MinION library preparation was amplified using the following primer sets (Table 2) . Firstly, a primer set based on Connecticut sequence NCBI FJ904716.1 was used to amplify Genotype I-lineage 1 viruses (e.g., Connecticut All rights reserved. No reuse allowed without permission......
Document: For Sanger sequencing amplicons synthesis, cDNA from MinION library preparation was amplified using the following primer sets (Table 2) . Firstly, a primer set based on Connecticut sequence NCBI FJ904716.1 was used to amplify Genotype I-lineage 1 viruses (e.g., Connecticut All rights reserved. No reuse allowed without permission.
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