Author: Salman L. Butt; Eric C. Erwood; Jian Zhang; Holly S. Sellers; Kelsey Young; Kevin K. Lahmers; James B. Stanton
Title: Real-time, MinION-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples Document date: 2019_5_10
ID: hxmk6gvr_46
Snippet: Finally, the seventh multi-isolate sample (#2) contained four isolates per the non-MinION results, and the AmpSeq detected two of those four isolates. These data show that a co-infection of multiple IBV lineages existed in the above-mentioned samples but a single RT-qPCR, or Sanger sequencing of a single clone may not have detected these co-infecting IBV lineages and required multiple assays to detect all the IBV types. However, the AmpSeq protoc.....
Document: Finally, the seventh multi-isolate sample (#2) contained four isolates per the non-MinION results, and the AmpSeq detected two of those four isolates. These data show that a co-infection of multiple IBV lineages existed in the above-mentioned samples but a single RT-qPCR, or Sanger sequencing of a single clone may not have detected these co-infecting IBV lineages and required multiple assays to detect all the IBV types. However, the AmpSeq protocol accurately detected multiple IBV lineages in 4 of 7 samples, with partial detection in two of the remaining three samples (Table 4 ). In samples which had the same IBV type, Blast search of consensus All rights reserved. No reuse allowed without permission.
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