Author: Haile, S.; Nikiforuk, A. M.; Pandoh, P. K.; Twa, D. D. W.; Smailus, D. E.; Nguyen, J.; Pleasance, S.; Wong, A.; Zhao, Y.; Eisler, D.; Moksa, M.; Cao, Q.; Wong, M.; Su, E.; Krzywinski, M.; Nelson, J. M. T.; Mungall, A. J.; Tsang, F.; Prentice, L. M.; Jassem, A.; Manges, A. R.; Jones, S. J. M.; Coope, R. J.; Prystajecky, N.; Marra, M. A.; Krajden, M.; Hirst, M.
Title: Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents Cord-id: wrjubbwd Document date: 2021_3_12
ID: wrjubbwd
Snippet: The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
Document: The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
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